Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-16 11:00:00
Date modified: 2016-10-17 01:23:21
ompT + PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010001_sequence (Version 1) |
Description
MHETase is the MHET(Mono(2-hydroxyethyl) terephthalate)-degrading enzyme first identified in Ideonella sakaiensis.This sequence is the E. coli K12 optimized DNA sequence for MHETase.
Notes
This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.Source
This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter ompT Tag RBS 34 PETase GGS Linker His-Tag Stop Codon Start Codon GGS Linker | 1,46 59,118 47,58 155,1024 1025,1060 1061,1078 1079,1084 59,61 119,154 | feature/promoter promoter tag feature/tag ribosome_entry_site feature/rbs CDS feature/protein feature/misc sequence_feature feature/tag tag stop_codon feature/stop start_codon feature/start sequence_feature feature/misc |