Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-20 11:00:00
Date modified: 2016-10-17 01:26:20
pelB + PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010002_sequence (Version 1) |
Description
This GGS linker is a linker sequence that neutrally connects two protein domains together.The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein.
Notes
The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.Source
This sequence is publicly known and was privately modified.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS 34 pelB Tag PETase GGS Linker His-Tag Stop Codon Start Codon GGS Linker | 1,46 47,58 59,124 161,1030 1031,1066 1067,1084 1085,1090 59,61 125,160 | feature/promoter promoter ribosome_entry_site feature/rbs feature/tag tag CDS feature/protein feature/misc sequence_feature feature/tag tag stop_codon feature/stop start_codon feature/start sequence_feature feature/misc |