Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-20 11:00:00
Date modified: 2016-10-08 01:03:50
YebF + PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010003_sequence (Version 1) |
Description
This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein. This is an alternate version of BBa_K2010002, but serves the same function.Notes
The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.Source
This sequence is publicly known and was privately modified.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS 34 YebF GGS Linker PETase His-Tag GGS Linker Start Codon Stop Codon | 1,46 47,58 59,412 413,448 449,1318 1355,1372 1319,1354 59,61 1373,1378 | promoter feature/promoter ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature CDS feature/protein feature/tag tag sequence_feature feature/misc start_codon feature/start stop_codon feature/stop |