Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-20 11:00:00
Date modified: 2016-10-08 01:23:25
PETase Composite (T7, His-Tag)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2010006_sequence (Version 1) |
Description
This composite part includes PETase regulated by a T7 promoter, a strong constitutive promoter, and also a his-tag for purification. Because of the T7 promoter, this part should be used in expression cells that are compatible with this promoter.Notes
Optimized for E. coli K12.Removed complexities for gBlock ordering.
Source
This part is composite of various parts from various sources. Refer to parts for details.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS 34 Start Codon ompT Tag Linker sfGFP Linker PETase GGS Linker His-Tag Stop Codon | 1,46 47,58 65,67 65,124 125,130 131,844 845,859 860,1729 1730,1765 1766,1783 1784,1789 | feature/promoter promoter ribosome_entry_site feature/rbs start_codon feature/start feature/tag tag feature/misc sequence_feature feature/protein CDS sequence_feature feature/misc feature/protein CDS sequence_feature feature/misc tag feature/tag feature/stop stop_codon |