Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boyao Zhang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 04:46:07
PcpcG2-B0034-PleD-His(6)Tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2012026_sequence (Version 1) |
Description
Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K592003).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.Notes
The expression of PleD can be activated under the control of green light. His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography.Source
PcpcG2 comes from the genome of Synechocystis PCC6803(Part BBa_K592003).PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)
| Access | Instance | Definition |
| public public public | BBa_K592003 BBa_B0034 BBa_K2012028 | BBa_K592003 BBa_B0034 BBa_K2012028 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592003 BBa_B0034 BBa_K2012028 | 1,238 247,258 265,1656 | BBa_K592003 BBa_B0034 BBa_K2012028 |