Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Przemyslaw Nuc
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-14 07:21:45
prha1-E15'UTR->htsfGFP
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014001_sequence (Version 1) |
Description
We provide a very efficient expression system: tightly controlled and giving a high level of protein expression upon induction by rhamnose.Notes
We were looking for the shortest, functional rhamnose induced promoter and for the best performing and universal 5'UTR sequence for several promoters of our multipromoter expression system.Source
Rhamnose induced promoter is a modified part of Escherichia coli genome. 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).| Sequence Annotation | Location | Component / Role(s) |
| E1 ht-sfGFP CRP binding site CRP binding site RhaS binding site -35 signal -10 signal 2nd RBS RBS HisTag | 136,174 177,924 1,21 32,53 54,70 100,105 124,129 150,158 162,167 175,204 | feature/misc sequence_feature feature/protein CDS feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs feature/tag tag |