Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 02:29:09
pxylS-E1_5'UTR->sfGFP
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014002_sequence (Version 1) |
Description
pxylS-E1_5'UTR construct assembly was performed based on XylS promoter (Bba_K1741009). XylS consist only XylA part of the wild-type xylose promoter version XylWT (Bba_K1741007).pxylS-E1_5'UTR has an E1 enhancer introduced into promoter???s 5???UTR. E1 is an RBS from gene 10 of bacteriophage T7. This promoter is induced by xylose (0.4%), which starts the transcription of the ORF.
Construct contains his-tagged sfGFP protein.
Notes
We started working on xylose promoters, because of the cheap inducer-xylose and potentially tight control of expression. Modification of E1-enhancer in 5'UTR gives the highest protein expression levels.Source
Xylose promoter's sequence comes from E.coli genome, E1 enhancer comes from gene 10 of bacteroophage T7.| Sequence Annotation | Location | Component / Role(s) |
| CRP binding site XylR binding site XylR binding site -35 signal -10 signal 5'UTR E1 2nd RBS RBS His-tag ht-sfGFP | 1,19 20,34 40,55 56,60 79,84 90,131 107,115 119,124 132,161 132,881 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site tag feature/tag CDS feature/protein |