Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 02:09:22
pBAD-M5'UTR->sfGFP
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2014003_sequence (Version 1) |
Description
Ara1-UTR promoter is made on the basis of two of our previous biobricks. Arashort1, which is an arabinose induced promoter without AraC factor (BBa_K1741000), was used as a base, because it is much shorter than wild type (araC-araBAD), but still provides the same, or even higher, protein expression. By using specially designed primers, we have deleted the original 5???UTR from Arashort1 and replaced it with synthetic 5???UTR, which was previously used in melibiose induced promoter- Mel2 (BBa_K1741004). This synthetic 5???UTR was built by removing potential secondary structures- which involved RBS. Furthermore, RBS has also been better positioned- 7 nt upstream from AUG start codon.Notes
We designed shortened arabinose induced promoter with modified 5'UTR to provide higher expression level.Source
Arabinose induced promoter pBAD is a fragment of E.coli K-12 genome. The original 5'UTR region is replaced by synthetic unstructured sequence with a well positioned RBS previously tested on melibiose promoter from E.coli K-12 in 2015.| Sequence Annotation | Location | Component / Role(s) |
| M5'UTR ht-sfGFP HisTag O1 region -10 signal O2 region i1-i2 CAP binding site -35 signal | 284,309 310,1059 310,339 158,179 269,273 1,16 205,247 203,213 245,249 | sequence_feature feature/misc CDS feature/protein tag feature/tag feature/operator operator feature/misc sequence_feature feature/operator operator non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site sequence_feature feature/misc |