Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 10:11:15
AraC-pBAD->sfGFP_W
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014006_sequence (Version 1) |
Description
We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| O2 region O1 region i1-i2 region -10 signal 5'UTR AraC CDS ht-sfGFP-W CAP binding site -35 signal His-Tag | 909,924 1066,1087 1113,1155 1177,1181 1191,1217 1,879 1218,1967 1111,1121 1153,1157 1218,1247 | operator feature/operator operator feature/operator feature/binding non_covalent_binding_site feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS feature/protein CDS feature/binding non_covalent_binding_site sequence_feature feature/misc tag feature/tag |