BBa_K2014009

BBa_K2014009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2014009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 10:32:27

pxylS-E1_5'UTR->sfGFP_B



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K2014009_sequence (Version 1)

Description

We were using xylS promoter to compare the influence of different ORFs structures coding for the same protein sfGFP.

Notes

We were looking for the best method to optimize gene expression in E.coli.

Source

The xylS promoter is a modified version of xylR-xylF-xylA1 fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.

Sequence Annotation Location Component / Role(s)
XylR binding site
XylR binding site
-35 signal
-10 signal
CRP binding site
5'UTR E1
ht-sfGFP-B
2nd RBS
RBS
His-Tag
20,34
40,55
56,60
79,84
1,19
90,131
132,881
107,115
119,124
132,161
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
feature/misc sequence_feature
feature/misc sequence_feature
feature/binding non_covalent_binding_site
feature/misc sequence_feature
feature/protein CDS
ribosome_entry_site feature/rbs
ribosome_entry_site feature/rbs
tag feature/tag
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2014009/1