Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 10:37:34
pxylS-E1_5'UTR->sfGFP_W
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2014010_sequence (Version 1) |
Description
We were using xylS promoter to compare the influence of different ORFs structures coding for the same protein sfGFP. The xylS promoter is a modified version of xylR-xylF-xylA promoter from E.coli K-12 genome.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
We used one of the sfGFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| XylR binding site XylR binding site -35 signal -10 signal CRP binding site 5'UTR E1 ht-sfGFP-W 2nd RBS RBS His-Tag | 20,34 40,55 56,60 79,84 1,19 90,131 132,881 107,115 119,124 132,161 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/misc sequence_feature feature/misc sequence_feature non_covalent_binding_site feature/binding feature/misc sequence_feature CDS feature/protein feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs tag feature/tag |