| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K2019003_sequence (Version 1)
|
Description
TorA-Cas9 is a fusion of the TorA signaling sequence to the N-terminus of staphylococcus aureus CRISPR associated protein 9 (saCas9). TorA utilizes the twin arginine translocation (tat) pathway to direct the fused protein to the periplasm of the cell. Therefore, by fusing Cas9 to the C-terminus of the signaling sequence, it should be directed to the periplasm of the cell.
Notes
Part BBa_K1012002 was ordered as a gblock with homology to the BBa_K2019000-BBa_R0040 expression system. BBa_K2019000-BBa_R0040 was linearized at the 5' end of saCas9. A 2 piece gibson assembly was then performed with the gblock and Cas9 expression backbone.
Source
The TorA sequence is from part BBa_K1012002 of the 2013 Dundee Team. The saCas9 sequence is from another Northwestern 2016 iGEM part, BBa_K2019000.
