| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K2020002_sequence (Version 1)
|
Description
This composite part consists of the promoter
BBa_R0010, the RBS
BBa_B0034, the newly created BioBrick part
BBa_K2020001 and the terminator
BBa_B0010. BioBrick BBa_K2020001 is a composite part itself and includes the secretion tag pelB (
BBaJ32015) and a subtilisin E gene optimized for E. coli codon usage (
BBa_K2020000). Once introduced into Escherichia coli, this BioBrick is able to produce subtilisin E and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
Notes
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.
Source
The sequences of the BioBrick parts were obtained through the Registry. The construct was ordered at IDT. add links
