Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gavin Sutton
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:36:16
T7 Expression Construct for tolR, Periplasmic Domain
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2022008_sequence (Version 1) |
Description
This part contains tolR, Periplasmic Domain (BBa_K2022002) under the control of a strong T7 promoter and RBS (both from BBa_K525998). Also included is double terminator (BBa_B0017).Notes
We opted to use a T7 promoter to express g3p, as in many expression strains of E. coli (those that are DE3), T7 polymerase is IPTG-inducible. This, ultimately, allows the control of tolR expression, which is critical when the protein has the potential to harm the cell if overexpressed to too great an extent (as one could imagine being the case with vesicle formation)Source
An assembly of BBa_K525998 - BBa_K2022002 - BBa_B0017| Access | Instance | Definition |
| public public public | BBa_K525998 BBa_K2022002 BBa_B0017 | pT7 BBa_K2022002 BBa_B0017 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K525998 BBa_K2022002 BBa_B0017 | 1,32 39,269 278,445 | pT7 BBa_K2022002 BBa_B0017 |