Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Coline Sivelle
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 01:00:04
Part expressing the dimerization protein FRB* and GFP11 (subunit of tripartite GFP)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2039001_sequence (Version 1) |
Description
This part is the complementary part of Bba_K2039000.The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria.
FRB* protein is linked to GFP 11.
The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
Sequence and Features
Notes
The mutations introduced are : T2098L, K2095P, W2101F.Source
Gblock| Access | Instance | Definition |
| public public public public | BBa_J23119 BBa_B0030 BBa_K2039004 BBa_B0015 | BBa_J23119 BBa_B0030 BBa_K2039004 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0030 BBa_K2039004 BBa_B0015 | 1,35 44,58 67,564 573,701 | BBa_J23119 BBa_B0030 BBa_K2039004 BBa_B0015 |