Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mathieu Hubert
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:19:06
Fusion Si4-cellulose-binding domain-B domain protein A
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2053002_sequence (Version 1) |
Description
It is a fusion protein between the phage displayed silica-binding peptide (Si4) developed by Naik et al (Journal of Nanoscience and Nanotechnology, Volume 2, Number 1, February 2002, pp. 95-100(6)), the Clostridium cellulovorans cellulose-binding domain of cellulose-binding protein A (CBPa) with the B domain of staphylococcus aureus protein A (BpA). This fusion protein is used to bind silica and antibodies to cellulose. We use these functions to functionalize a silica-cellulose composite-based patch into an immunodetection-based system.Notes
Polyhistidine tag (6 His) was added at the N-terminal end as well as linkers to join Si4 to CBPa and also CBPa to BpA. pET43.1a-derived ribosome-binding site (RBS) and T7 terminator were added.Source
Silica-binding peptide (Si4) was taken from iGEM Leeds 2013 (BBa_K1028000), cellulose-binding domain of cellulose-binding protein A was taken from iGEM Bielefeld-Germany 2012 team (BBa_K863110) and B domain of protein A was taken from iGEM Warsaw 2008 (BBa_K103003).| Sequence Annotation | Location | Component / Role(s) |
| pET43.1a RBS Start codon His-Tag TEV cleavage site Si4 Linker 1 CBPa Linker 2 BpA Stop codon Stop codon Terminator T7 | 1,36 23,28 37,39 40,57 58,78 79,123 124,159 160,465 466,501 502,723 724,726 727,729 730,776 | feature/scar feature/rbs ribosome_entry_site feature/start start_codon tag feature/tag feature/misc sequence_feature CDS feature/protein feature/protein CDS feature/protein CDS CDS feature/protein feature/protein CDS stop_codon feature/stop stop_codon feature/stop feature/dna sequence_feature |