Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: avi matityahu
Date created: 2016-08-22 11:00:00
Date modified: 2016-10-10 04:39:37
gRNA2 for editing F508 deletion
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K2061003_sequence (Version 1) |
Description
This plasmid contains the sequence coding for the second gRNA for editing the CFTR F508 deletion under U6 promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance.Notes
the part cloned between EcoRI and PstI sites.Source
synthetic production of the part| Sequence Annotation | Location | Component / Role(s) |
| EcorI site U6 Ex11 gRNA2 PAM gRNA scaffold seq PstI site | 1,6 7,324 326,345 346,348 349,425 466,471 | sequence_feature feature/misc feature/promoter promoter feature/cds CDS sequence_feature feature/misc CDS feature/cds feature/misc sequence_feature |