Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-31 11:00:00
Date modified: 2016-10-12 01:04:14
pTac+RiboJ+sfGFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2066024_sequence (Version 1) |
Description
This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. This part follows Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???). This part will be used in concert with K2066025 (pTac Ribozyme Chracterization Part ??? cI GFP) to determine the influence of the RiboJ insulator sequence on gene expression.Notes
This part was made for 2016 WM iGEM's Ribozyme characterization project.Source
The RBS, RiboJ and reporter sequences are from Lou et. al 2012. The UNS sequences are from Torella et. al 2013.| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K864400 BBa_K2066506 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K864400 BBa_K2066506 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 |