Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-19 05:30:24
pTAC sfGFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2066030_sequence (Version 1) |
Description
This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).This part follows Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part ??? cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
Source
Sequence follows Lou et al. (2012) and was synthesized using parts from the iGEM registry as well as UNS spacer sequences from Torella et. al (2013).| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K864400 BBa_K2066528 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K864400 BBa_K2066528 BBa_K2066510 BBa_K2066509 BBa_B0015 BBa_K2066019 |