Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-19 08:40:51
pTet GFP + TetR (weakened)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2066109_sequence (Version 1) |
Description
This part places both pTet GFP and it's repressor TetR on the same plasmid, which both reduces metabolic stress on transformed cells and helps limit the number of plasmids used by the circuit control toolbox. Placing all circuit control parts on a single plasmid will allow a workable number of plasmids to be used for the input circut to the circuit control toolbox circuit.Notes
This part contains a spacer which can be used to amplify either of the inserts to check for errors. This spacer sequence is derived from UNS6 from Torella et. al 2013Source
Parts are from registry with the exception of UNS backbone (WM16_iGEM universal primer) which comes from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public | BBa_K2066018 BBa_R0040 BBa_K2066529 BBa_B0034 BBa_K2066530 BBa_E0040 BBa_K2066529 BBa_B0015 BBa_K2066507 BBa_J23105 BBa_K2066529 BBa_B0031 BBa_K2066530 BBa_C0040 BBa_K2066529 BBa_B0015 BBa_K2066019 | BBa_K2066018 p(tetR) BBa_K2066529 BBa_B0034 BBa_K2066530 GFP BBa_K2066529 BBa_B0015 BBa_K2066507 BBa_J23105 BBa_K2066529 BBa_B0031 BBa_K2066530 tetR BBa_K2066529 BBa_B0015 BBa_K2066019 |