Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nithin Dharmaraj
Date created: 2016-09-06 11:00:00
Date modified: 2016-09-14 08:27:05
PCQuad 3.0 Wildtype
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2068000_sequence (Version 1) |
Description
The PCQuad gene codes for the production of one subunit of a protein cage. Each subunit is made up of a dimer protein(M1 Matrix) and a trimer protein(Bromoperoxidase) fused by an alpha helical linker. Expression of this subunit will lead to the self assembly of a tetrahedral structured protein cage. Protein cages, are self assembling supramolecular protein structures where multiple protein subunits interact non covalently to form a higher order structure. Our protein cage, PCquad, is a 12 subunit cage where each subunit is a fusion of two naturally oligomerizing proteins -- a trimer(Bromoperoxidase) and a dimer(M1 Matrix). The alpha helical fusion protein that links the trimer and dimer fixes them in a very specific orientation allowing for self assembly once subunits interact with each other.Notes
The fusion protein that links the dimer and trimer forms a 109.5 degree angle that makes self assembly more optimal.Source
This cage was designed by Dr. Todd Yeates at UCLA. It's subunits are a fusion of a naturally trimeric protein(Bromoperoxidase) and a naturally dimeric protein(M1 Matrix).| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter RBS PCQuad 3.0 6X His Tag Stop Codon Scar | 1,20 24,27 39,1400 1382,1400 1401,1405 33,38 | feature/promoter promoter feature/rbs ribosome_entry_site feature/BioBrick engineered_region tag feature/tag feature/stop stop_codon feature/scar |