Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WEI-CHIA HSU
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 04:34:48
kit1 fac-dex1+GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2073006_sequence (Version 1) |
Description
this part containBBa_J04500(IPTG promoter+RBS)+BBa_K2073000(fac-dex1)+BBa_E0040(GFP)+BBa_B0012(ter)
We transform this biobrick into E.coli strain BL21, and use IPTG promoter to control the fac-dex1 protein express.
When this engineered bacteria be induced by IPTG overnight (at least 12 hours), it would show GFP fluorescence.
Then we know the fac-dex1 is expressed by bacteria.
Notes
If bacteria show GFP fluorescence, we could know the fac-dex1 is successfully expressed.Source
BBa_J04500+BBa_K2073000+BBa_E0040+BBa_B0012| Access | Instance | Definition |
| public public public public | BBa_J04500 BBa_K2073000 BBa_E0040 BBa_B0012 | BBa_J04500 BBa_K2073000 GFP BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K2073000 BBa_E0040 BBa_B0012 | 1,220 227,1138 1145,1864 1873,1913 | BBa_J04500 BBa_K2073000 GFP BBa_B0012 |