Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WEI-CHIA HSU
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 04:34:57
fac-dex1 with IPTG promoter and ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2073008_sequence (Version 1) |
Description
This part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073000(fac-dex1)+BBa_B0012(ter).This biobrick can be transform into E.coli, then the E.coli can express fac-dex1.
fac-dex1 is a kind of fluoroacetate dehalogenase.
The enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate.
The reaction: a haloacetate + H2O ⇌ glycolate + a halide anion + H+
Notes
we can use IPTG to induce engineered bacteria expressing fac-dex1.Source
BBa_J04500(IPTG promoter+RBS)+BBa_K2073000(fac-dex1)+BBa_B0012(ter)| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_K2073000 BBa_B0012 | BBa_J04500 BBa_K2073000 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K2073000 BBa_B0012 | 1,220 227,1138 1147,1187 | BBa_J04500 BBa_K2073000 BBa_B0012 |