Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WEI-CHIA HSU
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-29 04:35:06
fac-dex3 with IPTG promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2073009_sequence (Version 1) |
Description
This part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_B0012(ter). This biobrick can be transform into E.coli, then the E.coli can express fac-dex3. fac-dex3 is a kind of fluoroacetate dehalogenase. The enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate. The reaction: a haloacetate + H2O ⇌ glycolate + a halide anion + H+Notes
we can use IPTG to induce engineered bacteria expressing fac-dex1.Source
BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_B0012(ter)| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_K2073001 BBa_B0012 | BBa_J04500 BBa_K2073001 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K2073001 BBa_B0012 | 1,220 227,1297 1306,1346 | BBa_J04500 BBa_K2073001 BBa_B0012 |