BBa_K2080012

BBa_K2080012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2080012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haojian Li

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 05:50:56

bax-sgRNA-34

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K2080012_sequence (Version 1)

Description

This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.

Notes

We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.

Source

From BBa_K2080003
BBa_K2080008

Components

Access	Instance	Definition
public	BBa_K2080003	BBa_K2080003
public	BBa_K2080008	BBa_K2080008

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2080003	1,366	BBa_K2080003
BBa_K2080008	375,741	BBa_K2080008

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2080012/1

Member of these Collections

• iGEM Parts Registry