Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liu iGEM 2016
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-15 08:35:43
mRFP1 regulated by pLIP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2095003_sequence (Version 1) |
Description
This composite part was designed by the Linkoping iGEM team 2016 to demonstrate the functionality of the light-inducible promoter, LIP, Biobrick (BBa_K2095000) A synthesised truncated version of the promotor of the light-inducible protein gene (LIP) in Dunaliella microalgae was set to control the expression of the mRFP1 reporter gene, obtained from BBa_E1010. This part is terminated by a RuBisCO terminator (BBa_K2095002).The part was created to evaluate function and potential leakage of LIP in C. reinhardtii. To provide this information, we planned to perform experiments in successfully transformed algae. This by measuring the RFP expression in transformed algae, stimulated by light of different intensities, compared to algae grown in complete darkness. Unfortunately, the transformation of this part into C. reinhardtii failed, and no results on BBa_K2095000 functionality were provided.
Notes
Used a truncated LIP promoter in order to optimize the promoters gene activating properties.Source
Truncated LIP promoter (BBa_K2095000) source: Dunaliella sp, RFP (BBa_E1010) source: Discosoma striata, RBCS2 terminator (BBa_K2095002) source: pOpt_mVenus_Hyg expression plasmid.| Access | Instance | Definition |
| public public public | BBa_K2095000 BBa_E1010 BBa_K2095002 | BBa_K2095000 mRFP1 BBa_K2095002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2095000 BBa_E1010 BBa_K2095002 | 1,305 312,1017 1026,1272 | BBa_K2095000 mRFP1 BBa_K2095002 |