BBa_K2110005

BBa_K2110005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2110005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Le He

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-15 06:00:42

TPA inducible RFP expression system

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K2110005_sequence (Version 1)

Description

PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. This expression system consists of the TPA inducible Leader sequence, the enhanced promoter PGK1(BBa_K2110012), the PETase gene(BBa_K2110800) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA so that this is a positive feedback system. This system is used in Saccharomyces cerevisiae.

Notes

/

Source

BBa_K2110012
BBa_K2110800
BBa_K2110000

Components

Access	Instance	Definition
public	BBa_K2110001	BBa_K2110001
public	BBa_K2110012	BBa_K2110012
public	BBa_E1010	mRFP1
public	BBa_K2110000	BBa_K2110000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2110001	1,96	BBa_K2110001
BBa_K2110012	105,885	BBa_K2110012
BBa_E1010	892,1597	mRFP1
BBa_K2110000	1606,1853	BBa_K2110000

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2110005/1

Member of these Collections

• //regulation/positive
• iGEM Parts Registry