Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fanglin Zhao
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-31 08:24:35
OP Pesticides Residue Degrading
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2112015_sequence (Version 1) |
Description
The plasmid could be co-transformed into E.coli BL21(DE3) with another plasmid containing a lacI coding sequence. Then with the inducing by IPTG (a compound), target protein could be expressed in high efficiency.T7 promoter is a strong promoter to launch the transcription procedure.
Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated.
His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily.
When??the??degradation??finishes,??ultraviolet??light??or??natural??light??is??given??to??shed??on??the??bacteria,??and??thus??the??RecA??repair??gene??inside??the??bacteria??is??activated.??The??product??of??this??gene??can??activate??RecA??(SOS)??promoter.??Thus??the??bacteria??begin??to??express??suicide??gene??ccdB,??which??leads??the??bacteria??to??dying??automatically??in??order??to??avoid??the??secondary??pollution??on??vegetables??and??fruits??posed??by??genetically??engineered??bacteria.
Notes
To avoid second pollution on vegetables posed by genetically engineered bacteria.Source
From other plasmid.| Access | Instance | Definition |
| public public public public | BBa_K2112011 BBa_K1667002 BBa_K1667003 BBa_K1667004 | BBa_K2112011 BBa_K1667002 BBa_K1667003 BBa_K1667004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2112011 BBa_K1667002 BBa_K1667003 BBa_K1667004 | 1,152 159,1226 1235,1473 1482,1826 | BBa_K2112011 BBa_K1667002 BBa_K1667003 BBa_K1667004 |