BBa_K2121010

BBa_K2121010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2121010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leslie Wang

Date created: 2016-10-20 11:00:00

Date modified: 2016-10-21 12:55:47

Rhamnose_Lambda_Beta_B5ZVM8

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2121010_sequence (Version 1)

Description

Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).

Notes

Expression fusion with native S. meliloti protein.

Source

Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.

Components

Access	Instance	Definition
public	BBa_K2121007	BBa_K2121007
public	BBa_K2121002	BBa_K2121002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2121007	1,45	BBa_K2121007
BBa_K2121002	52,1056	BBa_K2121002

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2121010/1

Member of these Collections

• iGEM Parts Registry