Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leslie Wang
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 12:59:37
Rhamnose_Lambda_Beta_F7X3D1
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2121012_sequence (Version 1) |
Description
Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).Notes
Expression fusion with native S. meliloti protein.Source
Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.| Access | Instance | Definition |
| public public | BBa_K2121007 BBa_K2121004 | BBa_K2121007 BBa_K2121004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2121007 BBa_K2121004 | 1,45 52,1002 | BBa_K2121007 BBa_K2121004 |