BBa_K2132004

BBa_K2132004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2132004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YIFAN CHEN
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 03:28:55

HydA with SpyCatcher, Histag and TEV site



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2132004_sequence (Version 1)

    Description

    This is a modified protein, we added Histag and TEV site to the N-terminal site of hydA, and SpyCatcher to the C-terminal site of hydA.

    Notes

    We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

    Source

    Clostridium acetobutylicum ATCC 824.

    Sequence Annotation Location Component / Role(s)
    6x Histag
    TEV site
    hydA
    SpyCatcher
    		7,24
    25,45
    46,1791
    1822,2166
    		tag feature/tag
    feature/misc sequence_feature
    CDS feature/cds
    tag feature/tag

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2132004/1
     

    Attachments

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