Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 05:02:45
yfp-bicistronic anti-meth antibody-cfp
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2141003_sequence (Version 1) |
Description
This construct consists one domain of anti-meth scFv antibody (termed as c1 in the project) conjugated with YFP, and the other domain of anti-meth scFv antibody (termed as c2 in the project) conjugated with CFP. Also, the construct has its own T7 promoter sequence in addition to the T7 promoter in the vector it is cloned into. This design constructed with the thought of separately producing the two domains of the anti-meth antibody which are conjugated with fluorescence proteins YFP and CFP, when the antibody domains came together in the presence of methamphetamine, it will give a FRET (f??rster resonance energy transfer) emission between CFP and YFP.Notes
Bicistronic construct is designed to obtain a FRET effect by the approach of two domain towards each other in the presence of methamphetamineSource
It is ordered via IDT sponsorship. Anti-meth scFv is coming from mus musculus. YFP and CFP are the common fluorescent proteins which are derivatives of GFP.| Sequence Annotation | Location | Component / Role(s) |
| RBS ATG YFP GS linker SacI restriction site C1 Stop codon XhoI restriction site T7 promoter RBS ATG C2 GS linker AclI restriction site CFP Stop codon | 1,39 40,42 44,705 706,717 718,723 724,1053 1054,1056 1057,1062 1063,1114 1115,1154 1155,1157 1158,1508 1509,1520 1521,1526 1527,2237 2238,2240 | ribosome_entry_site feature/rbs feature/start start_codon feature/cds CDS feature/misc sequence_feature sequence_feature feature/misc feature/cds CDS feature/stop stop_codon feature/misc sequence_feature feature/promoter promoter feature/rbs ribosome_entry_site start_codon feature/start feature/cds CDS sequence_feature feature/misc sequence_feature feature/misc CDS feature/cds stop_codon feature/stop |