Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:43:51
Coding sequence for Defensin with His6 and LPXTG-tag regulated by T7-promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2144007_sequence (Version 1) |
Description
Coding sequence for Defensin with His6 and LPXTG-tag regulated by T7-promoter. The function of Defensin is to lyse the pathogenic cell by permeabilizing the plasma membrane.The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein.
Notes
The LT-sequence was inserted with Overhang PCR.Source
-| Access | Instance | Definition |
| public public | BBa_K2144003 BBa_K2144009 | BBa_K2144003 BBa_K2144009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2144003 BBa_K2144009 | 1,200 209,259 | BBa_K2144003 BBa_K2144009 |