Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Xinyu Chen, Melody Wu, Katie Maruna
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-30 08:09:51
GFP/RFP reporter (Forward/Forward) with random 500bp spacer
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2145000_sequence (Version 1) |
Description
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.Notes
This is one part of a series of parts, each with different orientations of GFP and RFP.It was used to test the expression of fluorescence under different conditions of GFP and RFP.
Source
The GFP and RFP parts come from the iGEM GFP & RFP coding devices.| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 BBa_B0034 conserved BBa_E0040 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_I13522 BBa_R0010 CAP -35 -10 LacI start BBa_B0034 conserved BBa_E1010 mrfp1 barcode BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J04450 Golden Gate cloning scar 500 bp spacer Golden Gate cloning scar | 1,19 1,54 20,25 26,44 43,48 63,74 67,70 81,800 809,888 820,863 897,937 904,923 924,937 930,930 1,937 1446,1645 1534,1571 1582,1587 1606,1611 1611,1645 1618,1618 1654,1665 1658,1661 1672,2352 1672,2346 2353,2377 2386,2465 2397,2440 2474,2514 2481,2500 2501,2514 2507,2507 1446,2506 938,941 942,1441 1442,1445 | feature/binding non_covalent_binding_site engineered_region feature/BioBrick feature/promoter promoter non_covalent_binding_site feature/binding promoter feature/promoter engineered_region feature/BioBrick conserved_region feature/conserved feature/BioBrick engineered_region feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter feature/promoter promoter feature/binding non_covalent_binding_site feature/start start_codon engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/cds CDS feature/barcode engineered_tag engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick feature/scar feature/misc sequence_feature feature/scar |