Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ciara McCarthy
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-19 01:30:20
gRNA for CGG PAM
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K2148014_sequence (Version 1) |
Description
gRNA guiding Cas9 to trnE2-psbH intergenic region of the Chlamydomonas reinhardtii chloroplast genome. The part has a psaA promoter, coded as PROMOTER/5UTR/NTAG/CDS/CTAG Phytobrick.Notes
2 Gs added after TSS to stabilise gRNA, following advice of N. Patron (Earlham Institute)Source
Target sequence designed using Benchling software (https://benchling.com/crispr), scaffold sequence from Addgene (https://www.addgene.org/static/cms/files/hCRISPR_gRNA_Synthesis.pdf)| Sequence Annotation | Location | Component / Role(s) |
| psaA1 promoter target gRNA gRNA scaffold Phytobrick fusion site Phytobrick fusion site two extra Guanine for gRNA stability | 5,149 152,171 172,254 255,258 1,4 150,151 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc engineered_region feature/BioBrick engineered_region feature/BioBrick sequence_feature feature/misc |