Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianyi Huang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 07:14:21
pT7 TetX-GFP(fusion protein)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2150017_sequence (Version 1) |
Description
pT7+RBS+TetX-GFP(fusion protein)+DT.TetX and GFP are combined with a 3*GGGGS linker to form a fusion protein.
Notes
Expression level of tetX can be measured by the fluorescence intensity of GFP and the expression of tetracycline-degrading enzyme tetX can be amplified by T7 RNA polymerase.Source
pT7 promoter with RBS comes from BBa_K525998,DT from BBa_B0015.As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
| Access | Instance | Definition |
| public public public public | BBa_K2150031 BBa_B0034 BBa_K2150013 BBa_B0015 | pT7 BBa_B0034 tetX-GFP BBa_B0015 |