Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianyi Huang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 08:45:07
pCon+RBS+tetR+DT+pT7+pTet+RBS+GFP+RBS+T7RNAP+DT
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2150021_sequence (Version 1) |
Description
This part is a complete regulatory device sensitive to tetracycline.The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed be Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.
Notes
This part is used for logic verification. Whether green fluorescence is observed serves as an indicator whether the system works. In real use, substitute GFP for target gene.Source
pT7:PCR amplified from BBa_K525998,T7RNAP:PCR amplified from genome of BL21.pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, pTet from BBa_R0040, DT from BBa_B0015, GFP from BBa_E0040.