Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianyi Huang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 08:45:44
pCon+RBS+tetR+DT+pTet+RBS+T7RNP+Ter+RBS+pT7+GFP+DT
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2150023_sequence (Version 1) |
Description
This part is a complete regulatory device sensitive to tetracycline. The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no T7RNAP is produced, resulting in the inactivity of pT7. Thus, no GFP is expressed and no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of T7RNAP and activation of pT7 which further leads to the expression and amplification of GFP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. This part is used to test the tet switch and the tet-on/tet-off system.Notes
This part is used for logic verification. Whether green fluorescence is observed serves as an indicator whether the system works. In real use, substitute GFP for target gene.Source
pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, DT from BBa_B0015,PTet from BBa_R0040, pT7 from BBa_K525998, GFP from BBa_E0040.First terminator comes from BBa_B0013, while the second terminator from BBa_B0015.
T7RNAP is PCR amplified from genome of BL21.
| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_J23119 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_I2032 BBa_B0013 BBa_K2150031 BBa_B0034 BBa_E0040 BBa_B0015 | BBa_J23119 BBa_B0034 tetR BBa_B0015 p(tetR) BBa_B0034 BBa_I2032 BBa_B0013 pT7 BBa_B0034 GFP BBa_B0015 |