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BBa_K2150030

BBa_K2150030 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2150030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jianyi Huang

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-15 10:13:59

pCon+pTet+pLas+RBS+ydcD+DT+pCon+RBS+lasR+DT

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Details

Types	DnaRegion
Roles	engineered_region Device
Sequences	BBa_K2150030_sequence (Version 1)

Description

This part regulates the expression of antitoxin ydcD by adding or removing 3-oxo-dodecanoyl homoserine
lactone (3O-C12-HSL). LasR is a transcription factor and is constitutively expressed. It can couple with 3O-C12-HSL and activate pLas, thus producing antitoxin.

Notes

planning

Source

PLas from BBa_R0079,LasR from BBa_C0079,ydcD from BBa_K733012

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_J23119 BBa_R0040 BBa_R0079 BBa_B0034 BBa_K2150032 BBa_B0015 BBa_J23119 BBa_B0034 BBa_C0079 BBa_B0015	BBa_J23119 p(tetR) LasR+PAI BBa_B0034 ydcD BBa_B0015 BBa_J23119 BBa_B0034 lasr BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23119 BBa_R0040 BBa_R0079 BBa_B0034 BBa_K2150032 BBa_B0015 BBa_J23119 BBa_B0034 BBa_C0079 BBa_B0015	1,35 44,97 106,262 271,282 289,573 582,710 719,753 762,773 780,1560 1569,1697	BBa_J23119 p(tetR) LasR+PAI BBa_B0034 ydcD BBa_B0015 BBa_J23119 BBa_B0034 lasr BBa_B0015