Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Max Mustermann
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-17 01:05:29
Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2170000_sequence (Version 1) |
Description
Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA (CMV_BM40_BaP_NanoLuc_A3C5_EGFR TMD_mRuby_Strep_polyA_IRES_Kozak_IgKappa_BirA_StrepDEL_polyA)Notes
noneSource
assembly| Sequence Annotation | Location | Component / Role(s) |
| CMV T7 extended Kozak sequence BM40 signal peptide BAP NanoLuc A3C5 Tag poly G linker EGFR TMD EGFR stop transfer sequence poly G linker mRuby3 Strep TagII IRES (elF4G1) extended Kozak sequence Ig Kappa signal peptide BirA Strep TagII StepDEL KDEL (ER-retention signal polyA | 1,559 604,622 632,643 641,691 701,745 752,1261 1268,1300 1307,1336 1343,1411 1412,1438 1439,1468 1475,2182 2189,2212 2258,2597 2604,2616 2611,2672 2682,3641 3648,3672 3648,3680 3670,3680 3691,4171 | promoter feature/promoter promoter feature/promoter feature/misc sequence_feature feature/cds CDS feature/binding non_covalent_binding_site feature/protein CDS tag feature/tag feature/cds CDS CDS feature/cds feature/cds CDS CDS feature/cds feature/protein CDS feature/tag tag feature/rbs ribosome_entry_site sequence_feature feature/misc feature/cds CDS CDS feature/protein tag feature/tag feature/cds CDS feature/cds CDS feature/polya polyA_site |