Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margalit Mualem
Date created: 2016-09-26 11:00:00
Date modified: 2016-09-27 06:13:36
SNP for non-taster grapefruit juice bitterness+ gRNA
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2184032_sequence (Version 1) |
Description
Engineered SNP (Single Nucleotide Polymorphism) in favor of this reaction. This SNP enables identification of a certain taste out of the five known senses of taste, Each of the SNP???s that were selected Refer to people that unables identification of a certain taste out of the five known senses of taste.Using SNP segments ??? the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.
ThIs molecule WAS specially designed so that it was able to identify SNPs in specific allele flavor respectively and mediate CRISPR editing of the non-taste alleles only.
Notes
Engineered mutant SNP for non taster grapefruit juice bitterness taste by swich T with C base.Engineered gRNA BY Crispr.MIT.Edu.
Source
Human genomic for non taster seet taste| Access | Instance | Definition |
| public public public | BBa_K2184001 BBa_K2184001 BBa_K2184031 | BBa_K2184001 BBa_K2184001 BBa_K2184031 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2184001 BBa_K2184001 BBa_K2184031 | 1,20 29,48 29,209 | BBa_K2184001 BBa_K2184001 BBa_K2184031 |