Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Sandock
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:31
Salt Stress Activated Promoter Pro U and RBS+RFP+Term
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K220006_sequence (Version 1) |
Description
The ProU promoter (658 bp) was obtained from wild type K12 E. Coli Genomic DNA. Pro U controls transcription of the Pro U operon which contains three sub-units instrumental in bringing choline and proline into the cell. The promoter is most active during times of osmotic stress. Placing the proU promoter before the gene codes for red fluorescence protein with ribosomal binding site would enhance the level of red fluorescence expression under salt stress. We PCR amplified the gene proU with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.Reference:
T Annamalai, K Venkitanarayanan
Role of proP and proU in betaine uptake by Y. enterocolitica under cold and osmotic stress conditions
Notes
Length: 30 Fwd: 5'- AAT ACT AGT CTT TTT TCT GCG CAC CTC ACG -3'Length: 42 Rev: 5'- AAT ACT AGT GCA ATA GAA AGA TTC CTT TAT TTG TCT ATG TCG -3'
Source
ProU promoter is a gene isolated from K12 E. Coli Genomic DNA while the RFP part was taken from the registry| Access | Instance | Definition |
| public public | BBa_K220004 BBa_I13507 | BBa_K220004 BBa_I13507 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K220004 BBa_I13507 | 1,658 667,1527 | BBa_K220004 BBa_I13507 |