Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gao Rencheng
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:32
AND Gate LuxR-rbs(J44001)-T7ptag-AraC-SupD
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228043_sequence (Version 1) |
Description
{{PKU_Beijing2009_AND_GATE_FAMILY}}This is a AND GATE in the Ecoli stain DH5a. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
This gate is only one of nine gates(K228038-K228045), which are different in the RBS of T7ptag.
For more information:
refer to K228000(T7ptag) and K228001(SupD)
referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Source
PKU_Beijing iGEM 2009| Access | Instance | Definition |
| public public public public public | BBa_K228223 BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228012 | BBa_K228223 BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228223 BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228012 | 1,3883 3938,3949 4904,4983 4992,5032 3892,5314 | BBa_K228223 BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228012 |