BBa_K228043

BBa_K228043 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228043
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gao Rencheng
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:32

AND Gate LuxR-rbs(J44001)-T7ptag-AraC-SupD



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K228043_sequence (Version 1)

Description

{{PKU_Beijing2009_AND_GATE_FAMILY}}

This is a AND GATE in the Ecoli stain DH5a. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.

This gate is only one of nine gates(K228038-K228045), which are different in the RBS of T7ptag.

For more information:
refer to K228000(T7ptag) and K228001(SupD)
referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133

Source

PKU_Beijing iGEM 2009

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