Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Min
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34
OR Gate-GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K228861_sequence (Version 1) |
Description
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.Notes
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.Source
from 2009 part registry distribution & Peking University 2009 igem team| Access | Instance | Definition |
| public public public public public public | BBa_K228004 BBa_I746361 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012 | BBa_K228004 BBa_I746361 BBa_B0030 GFP BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228004 BBa_I746361 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012 | 1,1052 1061,1152 1161,1175 1182,1901 1910,1989 1998,2038 | BBa_K228004 BBa_I746361 BBa_B0030 GFP BBa_B0010 BBa_B0012 |