BBa_K228862

BBa_K228862 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228862
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoqian Zhang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34

salP-GFP



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K228862_sequence (Version 1)

Description

T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.

Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.

Source

part registery 2009 distribution & PKU 2009 igem team

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K228862/1