Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoqian Zhang
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:34
AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228870_sequence (Version 1) |
Description
{{PKU_Beijing2009_AND_GATE_FAMILY}}This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Source
It is made up of standard subparts from the partregistry.| Access | Instance | Definition |
| public public | BBa_K228851 BBa_K228861 | BBa_K228851 BBa_K228861 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228851 BBa_K228861 | 1,1333 1342,3379 | BBa_K228851 BBa_K228861 |