BBa_K235023

BBa_K235023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K235023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyliah Clarkson
Date created: 2009-09-02 11:00:00
Date modified: 2015-05-08 01:11:36

[K235018][K235020] (mCherry generator, pAra-controlled signal inversion)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K235023_sequence (Version 1)

Description

This BioBrick produced mCherry fluorescent protein (J06504) under the control of the λcI promoter. When the cell metabolises an inducer for the arabinose promoter (arabinose or an analogue), then the λcI repressor protein (C0051) will be produced. This will halt the production of mCherry.

This system is sensitive to the cellular production of λcI repressor protein. Use with caution in cell strains with arabinose-related sensitivity or insensitivity.

Notes

Constructed using three-antibiotic assembly. On plasmid pSB1C3.

Source

K235018+K235020

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K235023/1