BBa_K242900

BBa_K242900 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K242900
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Enrique Paz
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:37

[p(T3/T7)-GFP-(E3 + E9 toxins)]



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K242900_sequence (Version 1)

    Description

    This is a device for killing the cell if phage T3 or T7 is inside the cell.

    RNA pol of T3 and T7 recognize the promoter and transcribe the toxic domain of colicin E3 and E9.

    Also you transcribe LuxI, an AHL productor.

    We expect high level of transcription with this promoter and polymerase.

    Notes

    Until not expected, basal transcription of the toxins could be a problem. Those promoter recognized by T3 and T7 RNApol are not recognized by E.Coli -in theory-.

    Source

    composite

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K242900/1
     

    Attachments

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