Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Enrique Paz
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:37
[p(T3/T7)-GFP-(E3 + E9 toxins)]
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K242900_sequence (Version 1) |
Description
This is a device for killing the cell if phage T3 or T7 is inside the cell.RNA pol of T3 and T7 recognize the promoter and transcribe the toxic domain of colicin E3 and E9.
Also you transcribe LuxI, an AHL productor.
We expect high level of transcription with this promoter and polymerase.
Notes
Until not expected, basal transcription of the toxins could be a problem. Those promoter recognized by T3 and T7 RNApol are not recognized by E.Coli -in theory-.Source
composite| Access | Instance | Definition |
| public public public public public | BBa_K242101 BBa_K242200 BBa_K242201 BBa_C0261 BBa_B0015 | BBa_K242101 BBa_K242200 BBa_K242201 BBa_C0261 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K242101 BBa_K242200 BBa_K242201 BBa_C0261 BBa_B0015 | 1,841 850,1192 1201,1631 1640,2300 2309,2437 | BBa_K242101 BBa_K242200 BBa_K242201 BBa_C0261 BBa_B0015 |