Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:37
His-FluA-Split Linker-Fok_i
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K243010_sequence (Version 1) |
Description
These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.Notes
The choice of the linker, lipocalin tag and purification tag allows us serval different combinations.Source
Combined the parts by serval cloning steps.| Access | Instance | Definition |
| public public public public public public public | BBa_K157011 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243001 | His Scar 25 BBa_K157004 Scar 25 linker Scar 25 Fok_i |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K157011 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243001 | 1,18 19,24 25,546 547,552 553,603 604,609 610,1188 | His Scar 25 BBa_K157004 Scar 25 linker Scar 25 Fok_i |