Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:37
Strep-FluA-Middle Linker-Fok_a
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K243024_sequence (Version 1) |
Description
This combination of parts use the benefits of an Strep-tag and is linked with a FluroescineA tag. The MiddleLinker (GlySerGlyGly)x2 produce a certain distance between the FluA tag and the protein domain Fok_a.Notes
The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possiblities, which we had to prove to achieve the best combiantion.Source
Combined by serial clonig steps.| Access | Instance | Definition |
| public public public public public public public | BBa_K157012 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243000 | BBa_K157012 Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_a |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K157012 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243000 | 1,24 25,30 31,552 553,558 559,582 583,588 589,1167 | BBa_K157012 Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_a |